Description
The ViPrimePLUS Haemophilus ducreyi qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Haemophilus ducreyi genomes in clinical samples (e.g. ulcer and genital swabs).
Haemophilus ducreyi is a fastidious, Gram negative coccobacillus, which is another common cause of Chancroid, a painful genital ulcer disease, aside from T. pallidum, C. trachomatis and herpes simplex viruses. This sexually transmitted pathogen causes opportunistic infection by breaking the skin epithelial layer and then invading the epidermis, thus resulting in inflammation at the infected site. It eventually leads to ulceration. The ulcers and enlarged inguinal lymph nodes are painful and easily ruptured. Exposures to open sores are highly infectious. The infection is endemic in many developing countries with lower socioeconomic status and is somehow linked to elevated risk of HIV infections. Generally, Chancroid affects males predominantly. Treatment is via antibiotic administration, though some resistant strains have emerged.
ViPrimePLUS Haemophilus ducreyi qPCR Kit is intended for research use only.
Kit Component
- H. ducreyi Primers and Probe Mix (H. ducreyi PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. ulcer,genital swabs) |
| Gene Target | Exodeoxyribonuclease V, gamma subunit |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2029 | ViPrimePLUS Haemophilus ducreyi qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Haemophilus ducreyi qPCR Kit
