Description
The ViPrimePLUS Haemophilus influenzae qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Haemophilus influenzae genomes in clinical samples (e.g. nasopharyngeal swab, bronchoalveolar lavage and cerebrospinal fluid).
Haemophilus influenzae is a Gram negative coccobacillus that resides in the mucosal membrane of the nasopharynx. It is the common cause of the community acquired pneumonia (CAP), aside from Streptococcus pneumoniae. In addition to that, H. influenzae, S. pneumoniae and Neisseria meningitidis are responsible for most of the bacterial meningitis cases. H. influenzae is further classified into two types: 1) encapsulated, which includes the highly pathogenic H. influenzae b (Hib), and 2) non-capsulated, which causes non-invasive infections. Hib infection is more prevalent in children causing bacteremia and acute bacterial meningitis. The non-encapsulated type is found more commonly in *****s, causing pneumonia.
ViPrimePLUS Haemophilus influenzae qPCR Kit is intended for research use only.
Kit Component
- H. influenzae Primers and Probe Mix (H. influenzae PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. nasopharyngeal swab, bronchoalveolar lavage, cerebrospinal fluid) |
| Gene Target | Peptidoglycan-associated outer membrane lipoprotein P6 (OMPP6) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2030 | ViPrimePLUS Haemophilus influenzae qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Haemophilus influenzae qPCR Kit
