Description
The ViPrimePLUS Klebsiella pneumoniae qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Klebsiella pneumoniae genomes in clinical samples (e.g. throat swab, cerebrospinal fluid, sputum and blood).
In the Klebsiella genus, Klebsiella pneumoniae and Klebsiella oxytoca are the most clinically significant pathogens. K. pneumoniae is a Gram negative, rod bacterium, which is a part of the normal flora in the gastrointestinal tract. It colonizes the skin, pharynx and the urinary tract as well by evading the host defense system. Being an opportunistic pathogen, the infection is common among the elderly, immunocompromised patients and alcoholics, where the host immune system is weakened. The clinical manifestations include pneumonia (most common), meningitis, bacteremia, urinary tract infection, and liver abscess. In recent years, K. pneumoniae has become an important pathogen in nosocomial infections, being responsible for the 3-7% of all nosocomial bacterial infections. The emergence of carbapenem-resistant K. pneumoniae has limited the choice of treatment of the infection.
ViPrimePLUS Klebsiella pneumoniae qPCR Kit is intended for research use only.
Kit Component
- K. pneumoniae Primers and Probe Mix (K. pneumoniae PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. throat swab, cerebrospinal fluid, sputum and blood) |
| Gene Target | Outer membrane phosphate porin precursor (phoE) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2032 | ViPrimePLUS Klebsiella pneumoniae qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Klebsiella pneumoniae qPCR Kit
