Description
The ViPrimePLUS Lyme disease qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of the three bacteria causing Lyme disease genomes in clinical samples (e.g. blood, cerebrospinal fluid, skin biopsy).
Lyme disease is a deer tick-borne disease commonly found in North America and Europe. The causing agents include Borrelia burgdorferi, B. afzelli and B. garinii, which are Gram negative bacteria. The ticks at the nymphal stage are most likely to infect people with Lyme disease. Via tick bites, they transfer the bacteria residing in the salivary gland into the human host. The site infection will turn into bull eye rash, which may be accompanied by erythema migraine. Flu-like symptoms such as fever, swollen lymph nodes, headaches and joints pain may follow. If treatment is neglected, dissemination of the infection through the bloodstream may lead to neurological infection and development of chronic symptoms in the heart, lung, and joints. Treatment with antibiotics is usually applied.
ViPrimePLUS Lyme disease qPCR Kit is intended for research use only.
Kit Component
- Lyme Primers and Probe Mix (Lyme PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. blood, cerebrospinal fluid, skin biopsy) |
| Gene Target | RecA gene in the B. burgdorferi, B. garinii and B. afzelii genomes |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2037 | ViPrimePLUS Lyme disease qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Lyme disease qPCR Kit
