Description
The ViPrimePLUS Chlamydia trachomatis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Chlamydia trachomatis genomes in clinical samples (e.g. urogenital fluids and swabs).
Chlamydia trachomatis is a Gram negative, rod-shaped bacterium, which is recognized as the top sexually transmitted disease pathogen. Transmission is via sexual intercourse and during birth when passed from the infected mothers to the babies. Newborns contracted with chlamydia eye infection may develop pneumonia subsequently. Men are usually the asymptomatic carriers although some may develop urethritis, while women suffer more severe complications such as pelvic inflammation disease, ectopic pregnancy and infertility. Contaminated hands with genital fluids may spread the infection to the eyes, resulting in conjunctivitis or trachoma. Trachoma is the most common infectious cause of blindness, causing a major public health concern. Thus, early detection is necessary as disease prevention measure.
ViPrimePLUS Chlamydia trachomatis qPCR Kit is intended for research use only.
Kit Component
- C. trachomatis Primers and Probe Mix (C. trachomatis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material |
• Faecal samples • Food samples |
| Gene Target | Cryptic plasmid helicase (DnaB) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2012 | ViPrimePLUS Chlamydia trachomatis qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Chlamydia trachomatis qPCR Kit
