Description
The ViPrimePLUS Chlamydophila pneumoniae qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Chlamydophila pneumoniae genomes in clinical samples (e.g. respiratory fluids and swabs).
Chlamydophila pneumoniae is a Gram negative, obligated intracellular bacterium which infects the mucosal membrane of the respiratory tract, causing atypical pneumonia, bronchitis, sinusitis, laryngitis and pharyngitis. It is an atypical pathogen that causes 10-20% of the community-acquired pneumonia cases in *****s. Risk of infection in males is higher than females, which is probably attributed to smoking. An elderly person may suffer more severe complications from the infection than youths. Incubation period of the infections is 3-4 weeks with gradual onset, from milder upper respiratory tract symptoms, such as persistent coughing, malaise and fever, to more serious pneumonia and bronchitis development. Detection with real time PCR provides real and accurate diagnosis of the source of infection.
ViPrimePLUS Chlamydophila pneumoniae qPCR Kit is intended for research use only.
Kit Component
- C. pneumoniae Primers and Probe Mix (C. pneumoniae PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Cinical samples (e.g. respiratory fluids and swabs) |
| Gene Target | Major outer membrane protein (MOMP) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2013 | ViPrimePLUS Chlamydophila pneumoniae qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Chlamydophila pneumoniae qPCR Kit
