Description
The ViPrimePLUS Clostridium tetani qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Clostridium tetani genomes in wound swab, blood and faecal samples.
Clostridium spp. represents a group of anaerobic, spore-forming, Gram positive bacteria that infects the gastrointestinal tract, causing serious diarrhea, or the nervous system, causing neurological damage. Clostridium spp. has great survival mechanism because they sporulate under extreme conditions, but transform into infectious form when the environment turns favorable again. Clostridium tetani is responsible in causing tetanus in humans and horses. The organism enters the body through open wounds and releases an exotoxin, i.e. tetanospasmin which causes painful muscles spasms. This commonly affects the face and neck muscles, resulting in lockjaw or trismus and will eventually lead to respiratory failure if left untreated. Vaccination program is now being practiced in many developed and developing countries.
ViPrimePLUS Clostridium tetani qPCR Kit is intended for research use only.
Kit Component
- C. tetani Primers and Probe Mix (C. tetani PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. swab, blood, faecal) |
| Gene Target | Tetanus neurotoxin heavy chain 2 gene. |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2019 | ViPrimePLUS Clostridium tetani qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Clostridium tetani qPCR Kit
