Description
The ViPrimePLUS Bacillus anthracis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Bacillus anthracis genomes in clinical (e.g. nasopharyngeal and wound swab) or soil samples.
Bacillus anthracis is a Gram negative, rod-shaped bacterium that exists in both active growing form and dormant vegetative spores in soil. The infection causes anthrax, a lethal infection to both herbivorous mammals and humans. The hardiness of the spores in the environment allows effective aerosolization and dissemination, thus making it a potential biological weapon for global bioterrorism threat. Normally, transmission is through airborne or ingestion of infected animal products. There are 4 manifestations of the disease; 1) inhalation anthrax that is highly fatal if left untreated and a concerned cause of intentional aerosol attack, 2) cutaneous anthrax which is responsible for 95% of the reported cases and causes eschar, 3) gastrointestinal anthrax, and 4) the emerging injection related anthrax which infects mainly intravenous drug users.
ViPrimePLUS Bacillus anthracis qPCR Kit is intended for research use only.
Kit Component
- B. anthracis Primers and Probe Mix (B. anthracis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material |
• Clinical samples (e.g. nasopharyngeal and wound swab) • Soil samples |
| Gene Target | Hypothetical protein |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2003 | ViPrimePLUS Bacillus anthracis qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Bacillus anthracis qPCR Kit
