Description
The ViPrimePLUS Canine Distemper Virus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Canine Distemper Virus genome in blood, cerebrospinal fluids, pharyngeal swab, conjunctival swab and faecal samples.
Canine Distemper Virus (CDV) is a single-stranded RNA virus that infects dogs and wild animals such as wolves, foxes, raccoons and recently has been reported in the population of endangered Amur tigers, too. The virus is highly contagious and spreads via inhalation of contaminated bodily secretions and excretions. The infection resulted in various clinical symptoms including vomit, diarrhoea, fever, vision impairment and respiratory syndromes. Subsequently, the virus attacks the nervous system causing seizures, tremors and weakened limbs. Mortality rate can reach up to 50% and vaccines are not available against this virus. Early definitive diagnosis of the disease is essential to help owners and clinicians make decisions about continuing therapy versus euthanasia.
ViPrimePLUS Canine Distemper Virus RT-qPCR Kit is intended for research use only.
Kit Component
- CDV Primers and Probe Mix (CDV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Clinical samples (e.g. blood, cerebrospinal fluids, pharyngeal swab, conjunctival swab, faecal) |
| Gene Target | Nucleocapsid (N) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV2003 | ViPrimePLUS Canine Distemper Virus RT-qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Canine Distemper Virus RT-qPCR Kit
