Description
The ViPrimePLUS Chlamydophila felis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Chlamydophila felis genome in organ tissues such as nasal and ocular swab samples.
Chlamydophila felis is a Gram negative bacterium, which causes ocular disease in domestic cats. It infects the conjunctiva and is considered one of the major causes of conjunctivitis in cats. There are evidences of human infection which causes keratoconjunctivitis, however the risk of zoonosis outbreak is low. Being an obligated intracellular pathogen, it cannot survive outside of host and is transmitted via contact with ocular secretions. Clinical signs include watery discharge from eyes, red and swollen conjunctiva, fever, lethargy, weight loss and inappetence. Pneumonia can occur in infected kittens. Young kittens aged less than a year are more prone to infection. Treatment is usually with tetracycline and doxycycline. Vaccination should be considered for cats at risk of exposure to infection.
ViPrimePLUS Chlamydophila felis qPCR Kit is intended for research use only.
Kit Component
- C. felis Primers and Probe Mix (C. felis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Feline samples (e.g. nasal, ocular swab) |
| Gene Target | Major outer membrane protein (ompA gene) |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV2005 | ViPrimePLUS Chlamydophila felis qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Chlamydophila felis qPCR Kit
