Description
The ViPrimePLUS Feline Calicivirus RT-qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Feline Calicivirus genome in respiratory samples, conjunctival and oral swab, blood, skin scrapings and lung tissue.
Feline calicivirus (FCV) is a single stranded RNA virus which causes the normally self-limiting oral and upper respiratory tract disease in cats. The viral transmission can be via contact with nasal, oral and conjunctivitis fluids where the virus is shed. The ability of the virus to survive on the dry surface for several weeks contributes to the indirect transmission by contaminated cages and feed. Most of the cats can totally eliminate the virus, while some remain as carriers for life. The common clinical signs are oral ulceration, lesions in the joints that may cause limping, conjunctivitis, pneumonia, fever, excessive salivation, and edema. The indistinguishable symptoms especially from feline herpesvirus infection make clinical diagnosis difficult. In rare, severe cases, the virulent systemic strain of feline calicivirus (VS-FCV) infection may lead to systemic illnesses and hemorrhage, which is often fatal.
ViPrimePLUS Feline Calicivirus RT-qPCR Kit is intended for research use only.
Kit Component
- FCV Primers and Probe Mix (FCV PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Feline samples (e.g. respiratory samples, conjunctival and oral swab, blood, skin scrapings, lung tissue) |
| Gene Target | Open reading frame 1 (orf1) gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV2006 | ViPrimePLUS Feline Calicivirus RT-qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Feline Calicivirus RT-qPCR Kit
