Description
The ViPrimePLUS Microsporum canis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Microsporum canis genome in skin and scalp samples.
Microsporum canis (M. canis) is a dermatophyte which causes dermatophytosis in cats and dogs. It also causes superficial infections of the scalp (tinea capitis) in humans and ringworm in cats and dogs. Transmission of the fungus is via contact with infected animals or arthrospores contaminated environment. The hardy spore will survive for 2 years at harsh condition. After attaching to the skin, the spores germinate into hyphae which will grow on dead, superficial skin cells. The infection will lead to hypersensitive reaction on skin, causing inflammation. Fungus will move to uninfected skin in circular manner forming ringworm.
ViPrimePLUS Microsporum canis qPCR Kit is intended for research use only.
Kit Component
- M. canis Primers and Probe Mix (M. canis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Canine and feline samples (e.g. skin, scalp) |
| Gene Target | PQ-loop repeat protein gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV2011 | ViPrimePLUS Microsporum canis qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Microsporum canis qPCR Kit
