Description
The ViPrimePLUS Mycoplasma felis qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Mycoplasma felis genome in clinical conjunctiva samples.
Mycoplasmas are Gram negative microorganisms that have been isolated from many animal species. M. felis is a common parasite of cats, which resides in the upper respiratory and lower genital tract. It can be carried asymptomatically or cause conjunctivitis, respiratory diseases and polyarthritis in cats. In horse, the infection causes pneumonia. The early symptoms include serous discharge from affect eyes, swelling of the conjunctiva, hyperaemia and uncontrolled spasms or twitching of the eyelid. As infection progresses the discharge becomes more mucoid, possibly containing puss and the conjunctival swelling worsen.
ViPrimePLUS Mycoplasma felis qPCR Kit is intended for research use only.
Kit Component
- M. felis Primers and Probe Mix (M. felis PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | RNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material | Feline samples (e.g. conjunctiva) |
| Gene Target | PQ-loop repeat protein gene |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QV2012 | ViPrimePLUS Mycoplasma felis qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Mycoplasma felis qPCR Kit
