产品详情
简单介绍：
Bovine Bone Morphogenetic Protein 4 (BMP4) CLIA Kit
详情介绍：
Product Description
| Species Reactivity | Bos taurus; Bovine (Cattle) |
|---|---|
| Format | 96 well plate |
| Detection Range | 156.25-10000 pg/mL |
| Sensitivity | 6.25 pg/mL |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Time | 2 hours, 40min |
| UniProt | Q2KJH1 |
| Gene ID | 407216 |
| Gene Name | Bone Morphogenetic Protein 4 |
| Gene Aliases | BMP2B; BMP2-B; BMP2B1; ZYME; DVR4; Bone morphogenetic protein 2B |
| Application | CLIA |
| Assay Type | Sandwich |
| Detection Method | Chemiluminescent |
| Shipping Condition | Blue ice |
| Storage Instructions | -20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles. |
| Precaution Of Use | Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption. |
| Expiry Date | 8 months |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Bone Morphogenetic Protein 4 (BMP4). No significant cross-reactivity or interference between Bone Morphogenetic Protein 4 (BMP4) and analogues was observed. |
| Test Principle | The microplate provided in this kit has been pre-coated with an antibody specific to Bone Morphogenetic Protein 4 (BMP4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Bone Morphogenetic Protein 4 (BMP4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Bone Morphogenetic Protein 4 (BMP4) level in the sample or standard.; |
| Assay Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bone Morphogenetic Protein 4 (BMP4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| Restrictions | For research use only. Not for diagnostic procedures. |
| Quality Systems | The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities. |
| Research Area | Developmental science;Bone metabolism; |
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