产品详情
简单介绍：
Bovine Growth Hormone (GH) ELISA Kit
详情介绍：
Product Description
| Species Reactivity | Bos taurus; Bovine (Cattle) |
|---|---|
| Format | 96 well plate |
| Detection Range | 0.312-20 ng/mL |
| Sensitivity | 0.132 ng/mL |
| Sample Type | Serum, plasma, tissue homogenates and other biological fluids |
| Assay Time | 3 hours |
| Gene Name | Growth Hormone |
| Gene Aliases | GH1; GH-N; GHN; hGH-N; Somatotropin; Hygetropin; Jintropin; Kigtropin; Pituitary Growth Hormone; Growth Hormone, Normal |
| Application | ELISA |
| Assay Type | Sandwich |
| Detection Method | Colorimetric |
| Shipping Condition | Blue ice |
| Storage Instructions | -20 °C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles. |
| Precaution Of Use | The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption. |
| Expiry Date | 8 months |
| Specificity | This assay has high sensitivity and excellent specificity for detection of Growth Hormone (GH). No significant cross-reactivity or interference between Growth Hormone (GH) and analogues was observed. |
| Test Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Hormone (GH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Hormone (GH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Hormone (GH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Hormone (GH) in the samples is then determined by comparing the O.D. of the samples to the standard curve. |
| Assay Precision | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Hormone (GH) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level |
| Stability | The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end. |
| Restrictions | For research use only. Not for diagnostic procedures. |
| Quality Systems | The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities. |
| Research Area | Endocrinology;Neuro science;Hormone metabolism; |
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