dsGreen for Real-Time PCR, 100×
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 41010 | 1 mL | 310.00$ | in stock |
dsGreen, an analog of SYBR® Green I, is a very sensitive dsDNA detection dye. High sensitivity, and high selectivity for dsDNA allow to use dsGreen as a universal dsDNA detection reagent for qPCR. No need to use labeled probes to detect amplification with dsGreen – unlabeled primers are sufficient.
Unlike other preparations of dsGreen provided by Lumiprobe for gel staining purposes, this formulation is specially designed to be used in real-time PCR experiments. Specific features are:
- Concentration of the dye is optimized for qPCR and carefully adjusted for reproducible results from lot to lot.
- PCR tested preparation – quality guaranteed
- Low fluorescence background – high fluorescence intensity gain
Note: fluorescent properties of dsGreen bound to dsDNA below are taken from the following publication: Zipper, H.; Brunner, H.; Bernhagen, J.; Vitzthum, F. Investigations on DNA intercalation and surface binding by SYBR Green I, its structure determination and methodological implications. Nucleic Acids Res., 2004, 32, e103.
qPCR curves with dsGreen
Recommended protocol
qPCR with dsGreen or SYBR Green I
Customers also purchased with this product
Cyanine5.5 amine
Amino derivative of Cyanine5.5, a far red emitting fluorescent cyanine dye.Sulfo-Cyanine5.5 NHS ester
Water soluble, sulfonated, far-red sulfo-Cyanine5.5 dye in the form of NHS ester.Cyanine7.5 amine
Near infrared dye Cyanine7.5 with free amino group.General properties
| Appearance: | light orange solution |
| Quality control: | NMR 1H, HPLC-MS (95%), PCR testing |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
| MSDS: | Download |
Spectral properties
| Excitation maximum, nm: | 454 |
| ε, L?mol?1?cm?1: | 73000 |
| Emission maximum, nm: | 524 |
| Fluorescence quantum yield: | 0,8 |
Product citations
- Schmidt, N.; Kollewe, A.; Constantin, C.E.; Henrich, S.; Ritzau-Jost, A.; Bildl, W.; Saalbach, A.; Hallermann, S.; Kulik, A.; Fakler, B.; Schulte, U. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron, 2017, 96(4), 827–838.e9. doi: 10.1016/j.neuron.2017.09.038
- Revalde, J.L.; Li, Y.; Wijeratne, T.S.; Bugde, P.; Hawkins, B.C.; Rosengren, R.J.; Paxton, J.W. Curcumin and its cyclohexanone analogue inhibited human Equilibrative nucleoside transporter 1 (ENT1) in pancreatic cancer cells. European Journal of Pharmacology, 2017, 803, 167–173. doi: 10.1016/j.ejphar.2017.03.055
- Wu, Z.-G.; Jiang, W.; Chen, S.-L.; Mantri, N.; Tao, Z.-M.; Jiang, C.-X. Insights from the Cold Transcriptome and Metabolome ofDendrobium officinale: Global Reprogramming of Metabolic and Gene Regulation Networks during Cold Acclimation. Frontiers in Plant Science, 2016, 7, 1653. doi: 10.3389/fpls.2016.01653
- Brauer, V.S.; Stomp, M.; Bouvier, T.; Fouilland, E.; Leboulanger, C.; Confurius-Guns, V.; Weissing, F.J.; Stal, L.J.; Huisman, J.Competition and facilitation between the marine nitrogen-fixing cyanobacterium Cyanothece and its associated bacterial community.Frontiers in Microbiology, 2015, 5, 795. doi: 10.3389/fmicb.2014.00795
