| Product Details | |
| Product Name | Anti-Histone H3.3 G34W Rabbit Monoclonal Antibody [RM263] |
| Product Description | Rabbit monoclonal to Histone H3.3 G34W; Histone H3.3 G34W Mutant |
| Catalog No. | 31-1145-00 |
| Clone Name | RM263 |
| Product Data Sheet | Anti-Histone H3.3 G34W Mutant Rabbit Monoclonal Antibody Data Sheet RM263 |
| Specificity | RM263 reacts to the Histone H3.3 G34W mutant. No cross reactivity with wild type Histone H3.3 . |
| Immunogen | A peptide corresponding to Histone H3.3 G34W mutant |
| Application | Western Blot ELISA Immunohistochemistry Immunocytochemistry Chromatin IP |
| Species Reactivity | All |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 50 ug |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.5 ug/mL - 2 ug/mL; Immunohistochemistry (IHC): 0.5 – 5 ug/mL; Immunocytochemistry (ICC): 0.5 – 5 ug/mL. |
| References for Histone H3.3 G34W Mutant antibody [RM263] | Yamamoto H.et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017)10.1016/j.humpath.2017.11.020 IHC; Human. Read more (PubMed: 29241742) Kato, I. et alGiant Cell Tumours of Bone Treated with Denosumab: Histologic, Immunohistochemical, and H3F3A Mutation AnalysesHistopathology 10.1111/his.13448.(2017). IHC-P; Human. IF; Human Read more (PubMed: 29206281) Luke J. et al. H3F3A mutation in giant cell tumour of the bone is detected by immunohistochemistry using a monoclonal antibody against the G34W mutated site of the histone H3.3 variant.Histopathology 10.1111/his.13190.(2017). IHC-P; Human. Read more (PubMed: 28211081) Amary F. et al. H3F3A (Histone 3.3) G34W Immunohistochemistry: A Reliable Marker Defining Benign and Malignant Giant Cell Tumor of Bone.AM J Surg Pathol.10.1097/PAS.0000000000000859. (2017). IHC-P; Human. Read more (PubMed: 28505000) |
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| Product Images | |
 | Western Blot analysis of cell lysates prepared from |
 | Immunohistochemical staining of formalin fixed and |
 | Immunohistochemical staining of Giant Cell Bone Image courtesy of Adrienne Flanagan, Department |
 | Immunohistochemical staining of Giant Cell Bone Image courtesy of Adrienne Flanagan, Department |
