| Product Details | |
| Product Name | Anti-Acetyl-Histone H3 (Lys14) Rabbit Monoclonal Antibody [RM130] |
| Product Description | Rabbit monoclonal to Acetylated Histone H3 Lysine 14 (K14ac), H3K14ac, Histone H3 (K14 acetylation) |
| Catalog No. | 31-1032-00 |
| Clone Name | RM130 |
| Product Data Sheet | Anti-Acetyl-Histone H3 Lys14 Rabbit Monoclonal Antibody Data Sheet RM130 |
| Specificity | RM130 reacts to Histone H3 acetylated at Lysine 14 (K14ac), and is not affected by the modification of neighboring amino acids. No cross reactivity with acetylated Lysine 4 (K4ac), Lysine 9 (K9ac), Lysine 18 (K18ac), Lysine 23 (K23ac), Lysine 27 (K27ac), Lysine 36 (K36ac), or lysine 79 (K79ac) in histone H3. |
| Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys14) |
| Application | Western Blot (WB) Immunocytochemistry (ICC) Chromatin IP (ChIP) ELISA Multiplex |
| Species Reactivity | Human Vertebrates |
| Conjugate | None |
| Intended Use | Research Use Only |
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| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 100 ug |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 ug/mL - 2 ug/mL; ICC: 0.5 ug/mL - 2 ug/mL; ChIP: 2 ug/mL- 10 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.1 ug/mL – 0.5 ug/mL. |
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| Product Images | |
 | RM130 specifically reacts to Histone H3 acetylated |
 | The binding specificity of RM130 to Histone H3 |
 | Western Blot of acid extracts from HeLa cells |
 | ChIP performed on HeLa cells using H3K14ac |
 | Immunocytochemistry of HeLa cells treated with |
