Product Details | |
Product Name | Anti-Acetyl-Histone H3 (Lys9) Rabbit Monoclonal Antibody [RM161] |
Product Description | Rabbit monoclonal to Acetylated Histone H3 Lysine 9 (K9ac), H3K9ac, Histone H3 (K9 acetylation) |
Catalog No. | 31-1054-00 |
Clone Name | RM161 |
Product Data Sheet | Anti-Acetyl-Histone H3 Lys9 Rabbit Monoclonal Antibody Data Sheet RM161 |
Specificity | RM161 reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3. |
Immunogen | An acetyl-peptide corresponding to Acetyl-Histone H3 (Lys9). |
Application | Western Blot (WB) Chromatin IP (ChIP) Immunocytochemistry (ICC) Immunohistochemistry (IHC) ELISA Multiplex |
Species Reactivity | Human Vertebrates |
Conjugate | None |
Intended Use | Research Use Only |
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Properties | |
Form | Liquid |
Storage Instructions | Store at -20.0°C |
Stability | Stable for 1 Year at -20.0°C from date of receipt |
Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
Volume/Size | 100 ug |
Concentration | 1.0 mg/mL |
Purity | Protein A affinity purified from an animal origin–free culture supernatant |
Clone Type | Monoclonal |
Isotype | Rabbit IgG |
Usage | Western Blot: 0.25 ug/mL - 1 ug/mL; ICC: 0.5 ug/mL - 2 ug/mL; ChIP: 2 ug/mL-10 ug/mL; IHC: 0.1 ug/mL – 1 ug/mL; ELISA: 0.2 ug/mL - 1 ug/mL; Multiplex: 0.05 ug/mL – 0.5 ug/mL. |
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References for Acetyl-Histone H3 (Lys9) antibody [RM161] | Li Y. et al. Activation of mutant TERT promoter by RAS-ERK signaling is a key step in malignant progression of BRAF-mutant human melanomas. Proc Natl Acad Sci USA. 113(50):14402-14407. ChIP; Human. Read more (PubMed: 27911794) |
Product Images | |
RM161 specifically reacts to Histone H3 acetylated |
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Western Blot of acid extracts from HeLa cells |
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Sandwich ELISA against acetylated histone H3 at Lys 9 |
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ChIP performed on HeLa cells using H3K9ac antibody (RM161, |
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Immunocytochemistry of HeLa cells treated with |
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Immunohistochemistry staining of HepG2 cells using |