| Product Details | |
| Product Name | Anti-Human IgG1 Rabbit Monoclonal Antibody [RM117] |
| Product Description | Rabbit monoclonal to Human IgG1 |
| Catalog No. | 31-1019-00 |
| Clone Name | RM117 |
| Product Data Sheet | Anti-Human IgG1 Rabbit Monoclonal Antibody Data Sheet RM117 |
| Specificity | RM117 reacts to the heavy chain of human IgG1. RM117 does not cross react to any other IgG subclasses (IgG2, IgG3, or IgG4), and shows no cross reactivity to IgM, IgA, IgD, IgE. RM117 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Peptide corresponding to the hinge region of Human IgG1 |
| Application | Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA |
| Species Reactivity | Human |
| Conjugate | None |
| Intended Use | Research Use Only |
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| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100ug |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL. |
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| Product Images | |
 | Detection of human IgG1 in monkey serum, using |
 | Sandwich ELISA using RM117 as the capture |
 | ELISA showing RM117 reacts only to human IgG1, |
 | A titer ELISA using RM117. The plate was coated |
 | ELISA showing RM117 does not react to monkey |
 | Immunohistochemical staining of FFPE Tonsil tissue Image courtesy of Shaomin Hu, MD, PhD, |
