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| Product Details | |
| Product Name | Anti-Human IgG3 Rabbit Monoclonal Antibody [RM119] |
| Product Description | Rabbit monoclonal to Human IgG3 |
| Catalog No. | 31-1021-00 |
| Clone Name | RM119 |
| Product Data Sheet | Anti-Human IgG3 Rabbit Monoclonal Antibody Data Sheet RM119 |
| Specificity | RM119 reacts to the heavy chain of human IgG3, RM119 does not cross react to any other IgG subclasses (IgG1, IgG2, or IgG4), and shows no cross reactivity IgM, IgA, IgD, IgE. RM119 does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| Immunogen | Human IgG3 |
| Application | Immunohistochemistry (IHC) Flow Cytometry (FC) Immunocytochemistry (ICC) ELISA |
| Species Reactivity | Human |
| Conjugate | None |
| Intended Use | Research Use Only |
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| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Item Size | 100ug |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | ELISA: 50ng/well – 200ng/well (for Capture); 0.05ug/mL – 0.2ug/mL (for Detection); Immunocytochemistry (ICC): 0.5ug/mL-2ug/mL; Immunohistochemistry (IHC): 0.5ug/mL-2ug/mL. |
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| Product Images | |
 | Detection of human IgG3 in monkey serum. |
 | Sandwich ELISA using RM119 as the capture antibody |
 | ELISA showing RM119 reacts only to human IgG3, |
 | A titer ELISA using RM119. The plate was coated |
 | Immunohistochemical staining of formalin fixed |
 | ELISA showing RM119 does not react to monkey |
 | Immunohistochemical staining of FFPE Tonsil tissue Image courtesy of Shaomin Hu, MD, PhD, |
