PerCP - anti Mouse TER-119 - TER-119
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103,00 € tax excl.
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265,00 € tax excl.
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Product Description
- TER-119
- Rat IgG2b
- Flow Cytometry
- PerCP
- 0.2 mg/ml
- Mouse Fetal Liver
- Mouse
- The reagent is provided in aqueous buffered solution containing protein stabilizer, and ≤0.09% sodium azide (NaN3)
- RUO
- Erythroid Cell, clone TER-119, is a mAb intended for RUO in the identification/enumeration of lymph that express Erythroid Cell. This reagent is effective for direct IF staining of mouse sample for FCM analysis using ≤1 μg/106 cells.
- Liquid
- Store in the dark at 2-8 °C
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TER-119 reacts in ***** mice with mature erythrocytes, 20±25% of bone marrow cells and 2±3% of spleen cells but not with thymocytes nor lymph node cells. In fetal haematopoietic tissues, 30±40% of d 10 yolk sac cells, 80±90% of d 14 fetal liver cells and 40±50% of newborn liver cells were reactive with TER-119. TER-1191 cells in ***** bone marrow expressed significant levels of CD45 but not myeloid (Mac-1, Gr-1) or B-cell (B220) markers. Morphological examination and haematopoietic colony-forming assays for isolated TER- 1191 cells revealed that TER-119 reacts with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte, but not with cells showing typical erythroid blast-forming unit (BFU-E) and erythroid colonyforming unit (CFU-E) activities. Erythroleukaemia cell lines do not express the TER-119 antigen even after stimulation with dimethylsulphoxide. TER-119 immunoprecipitated protein bands with molecular masses of 110 kDa, 60 kDa, 52 kDa and 32 kDa from erythrocyte membrane, whereas only a 52-kDa band was detected by TER-119 in Western blot analysis. Further molecular and cellular analyses indicated that the TER-119 antigen is a molecule associated with cell-surface glycophorin A but not with glycophorin A itself.
- Ly-76
- 104231
- 52 kD
