EVB101- Electro Competent E. coli B strain for in vivo biotinylation
SKU:
EVB101
$195.00
产品详情
简单介绍：
EVB101- Electro Competent E. coli B strain for in vivo biotinylation
详情介绍：
AVB101 cells made competent for uptake of plasmid DNA by electroporation. Efficiency of transformation is >1010 transformants per µg of supercoiled plasmid DNA.
Quantity: 10 vials, 50 μl per vial Store at -80°C.
Strain EVB101, an electro-competent E. coli B strain (hsdR, lon11, sulA1), contains pBirAcm , an IPTG inducible plasmid containing the BirA gene engineered into pACYC184. It is compatible with most cloning vectors and is maintained with chloramphenicol (10 μg/ml). This strain is recommended for protein expression because of its robust growth and the absence of the OmpT and Lon proteases.
At the time of induction, Biotin should be added at a concentration of 50 μM. The pAN and pAC vectors containing the AviTagTM require ampicillin (100 μg/ml).
相关文章
