Details
Host: Rabbit
Isotype: IgG
Class: Polyclonal
Immunogen: Synthetic peptide corresponding to Human c-Myc protein
Applications:
Red-colored affinity matrix consists of affinity purified anti-c-Myc rabbit polyclonal antibody covalently attached to red beaded agarose (Separopore®). The c-Myc tag corresponds to amino acid residues 410-419 (EQKLISEEDL) of the human c-Myc protein. Anti-cMyc Red-Separopore® 4B-CL is convenient for quantitative capturing of antigen/antibody complexes in immunoprecipitation, greatly reduces the risk of pellet loss. Comparable performance to non-colored affinity gels, but allows the user to easily differentiate pellet from supernatant. This results in greater consistency and more accurate data due to less sample losses. Anti-cMyc Red-Separopore® 4B-CL is reliable tool for affinity capture (immunoprecipitation) of N- or C-terminal c-Myc-tagged fusion proteins from E. coli or mammalian cells.
Red Separopore ® Technical Specifications:
- Matrix: Separopore® 4B-CL (crosslinked agarose beads, 4%)
- Concentration: 25 mg Red Separopore ® per mL of buffer
- Particle size range: 52 – 165 µm
- Ligand coupling method: CNBr activation
- Binding capacity: approximately 50 µg/ml drained gel
- Exclusion limit: approximately 2 x 107
- pH stability: 3 – 9 (for use), 2 – 10 (for cleaning)
- Supplied as 50% suspension in PBS, containing 50% glycerol.
Additional Information
Product # | V-2515 |
---|---|
Appearance | Liquid Suspension |
Storage Temp | 4°C |