产品详情
简单介绍：
Anti-PCNA Antibody
详情介绍：
Overview
| Name: | Anti-PCNA Antibody See all PCNA primary antibodies
|
| Description: | Mouse monoclonal (PC10) antibody to PCNA. |
| Applications: | IHC |
| Reactivity: | Human, Rat |
| Immunogen: | Recombinant rat PCNA (Protein A expression vector pR1T2T). |
| Host: | Mouse |
| Clonality: | Monoclonal |
| Clone: | PC10 |
| Isotype: | IgG2a |
| Conjugate: | Unconjugated |
| Product Form: | Liquid |
| Formulation: | Supplied in Phosphate Buffered Saline, pH 7.3, with 0.09% Sodium Azide. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
Target
| Function: | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity). |
| Sequence Similarities: | Belongs to the PCNA family. |
| Post-Translational Modification: | Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA (By similarity). |
| Cellular Location: | Nucleus. Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. Colocalizes with CREBBP, EP300 and POLD1 to sites of DNA damage (By similarity). |
| Database Links: |
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| Synonyms: | |
| Information: | Target information shown above is from the UniProt Consortium. |
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