企业信息
17
  • 入驻时间: 2008-08-20
  • 联系人:客户经理
  • 电话:400-968-7988
  • 联系时,请说明易展网看到的
  • Email:fuwu@bioleaf.com
产品详情
  • 产品名称:Anti-His Tag Antibody

  • 产品型号:
  • 产品厂商:其它品牌
  • 产品价格:155
  • 折扣价格:155
  • 产品文档:
你添加了1件商品 查看购物车
简单介绍:
Anti-His Tag Antibody
详情介绍:
Name: Anti-His Tag Antibody
See all His Tag primary antibodies
Description: Mouse monoclonal (HIS.H8) antibody to His Tag.
Specificity: Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e. reacts with N-terminal, C-terminal or internal 6x His-tags).
Applications: Dot, ELISA, IP, IS, WB
Immunogen: HHHHHH (6x His) synthetic peptide conjugated to KLH.
Host: Mouse
Clonality: Monoclonal
Clone: HIS.H8
Isotype: IgG2b
Conjugate: Unconjugated
Purification: Protein A affinity chromatography from mouse ascites fluid.
Concentration: 1 mg/ml
Product Form: Liquid
Formulation: Supplied in 10mM Phosphate Buffered Saline, pH 7.20, with 0.05% Sodium Azide.
Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Enlarge Image

Immunofluorescence (red) with Anti-His Tag Antibody on His-tag fusion protein in HEK293 cells. Left hand panel: untransfected control. Right hand panel: transfected.
Counterstained with DAPI (blue).

Enlarge Image

Standard ladder containing five different His-tagged proteins; untransfected (1), HEK293 cells transferred with His-tagged protein vector (2).

Enlarge Image

Comparison between Anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5µg/ml).

Enlarge Image

Western blot analysis of a 6x His-tagged protein was performed by loading 15µg of cell lysates from SF9 cells expressing an 11kD 6x-His tagged protein per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane, and blocked in blocking buffer for 1 hour at room temperature. The membrane was probed with Anti-His Tag Antibody at a dilution of 1:500 overnight at 4 degrees celsius, washed in TBST, and probed with an Anti-Mouse IgG secondary antibody conjugated to a near-IR dye. Results were visualized using a near-IR imager.
标题:
内容:
联系人:
联系电话:
Email:
公司名称:
联系地址:
 
 
注:1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!
相关文章

沪公网安备 31011202007337号