产品详情
简单介绍：
Anti-His Tag Antibody
详情介绍：
Overview
| Name: | Anti-His Tag Antibody See all His Tag primary antibodies
|
| Description: | Mouse monoclonal (HIS.H8) antibody to His Tag. |
| Specificity: | Recognizes the epitope of 6x His-tags encoded by many commercially available vectors, regardless of the tag's location in the fusion protein sequences (i.e. reacts with N-terminal, C-terminal or internal 6x His-tags). |
| Applications: | Dot, ELISA, IP, IS, WB |
| Immunogen: | HHHHHH (6x His) synthetic peptide conjugated to KLH. |
| Host: | Mouse |
| Clonality: | Monoclonal |
| Clone: | HIS.H8 |
| Isotype: | IgG2b |
| Conjugate: | Unconjugated |
| Purification: | Protein A affinity chromatography from mouse ascites fluid. |
| Concentration: | 1 mg/ml |
| Product Form: | Liquid |
| Formulation: | Supplied in 10mM Phosphate Buffered Saline, pH 7.20, with 0.05% Sodium Azide. |
| Storage: | Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. |
Images
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Immunofluorescence (red) with Anti-His Tag Antibody on His-tag fusion protein in HEK293 cells. Left hand panel: untransfected control. Right hand panel: transfected.
Counterstained with DAPI (blue).
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Standard ladder containing five different His-tagged proteins; untransfected (1), HEK293 cells transferred with His-tagged protein vector (2).
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Comparison between Anti-His tag (HIS.H8 / EH158) mAb with 2 different vendor Abs, probed against a standard ladder containing five different His-tagged proteins. All Ab dilutions are 1:2000 (0.5µg/ml).
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Western blot analysis of a 6x His-tagged protein was performed by loading 15µg of cell lysates from SF9 cells expressing an 11kD 6x-His tagged protein per well onto an SDS-PAGE gel. Proteins were transferred to a nitrocellulose membrane, and blocked in blocking buffer for 1 hour at room temperature. The membrane was probed with Anti-His Tag Antibody at a dilution of 1:500 overnight at 4 degrees celsius, washed in TBST, and probed with an Anti-Mouse IgG secondary antibody conjugated to a near-IR dye. Results were visualized using a near-IR imager.
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