产品详情
简单介绍：
Anti-Bcl-2 (P65) Antibody
详情介绍：
Overview
| Name: | Anti-Bcl-2 (P65) Antibody See all Bcl-2 primary antibodies
|
| Description: | Rabbit polyclonal antibody to Bcl-2 (P65) |
| Specificity: | Bcl-2 (P65) pAb detects endogenous levels of Bcl-2 protein. |
| Applications: | WB, IHC, IF |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Synthetic peptide, corresponding to amino acids 31-80 of Human Bcl-2. |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Conjugate: | Unconjugated |
| Molecular Weight: | ~ 26 kDa |
| Purity: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Product Form: | 1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2. |
Target
| Function: | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). |
| Tissue Specificity: | Expressed in a variety of tissues. |
| Sequence Similarities: | Belongs to the Bcl-2 family. |
| Post-Translational Modification: | Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). |
| Cellular Location: | Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. |
| Database Links: |
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| Synonyms: | |
| Information: | Target information shown above is from the UniProt Consortium. |
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