产品详情
简单介绍：
Anti-Fyn Antibody
详情介绍：
Overview
| Name: | Anti-Fyn Antibody See all Fyn primary antibodies
|
| Description: | Mouse monoclonal (FYN-01) antibody to Fyn. |
| Specificity: | The antibody FYN-01 reacts with Fyn, a 59 kDa non-receptor Src-family protein tyrosine kinase. |
| Applications: | IP, WB, IHC-P, ICC |
| Reactivity: | Mouse, Human |
| Immunogen: | Bacterially expressed recombinant fragment of human Fyn (aa 7-176). |
| Host: | Mouse |
| Clonality: | Monoclonal |
| Clone: | FYN-01 |
| Isotype: | IgG2b |
| Conjugate: | Unconjugated |
| Purification: | Protein A chromatography. |
| Concentration: | 1 mg/ml |
| Purity: | > 95% (by SDS-PAGE) |
| Product Form: | Liquid |
| Formulation: | Supplied in Phosphate Buffered Saline, pH 7.4, with 15mM Sodium Azide. |
| Storage: | Store at 2-8°C. |
Target
| Function: | Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1 (By similarity). |
| Tissue Specificity: | Isoform 1 is highly expressed in the brain, isoform 2 is expressed in cells of hemopoietic lineages, especially T-lymphocytes. |
| Sequence Similarities: | Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. |
| Post-Translational Modification: | Autophosphorylated at Tyr-420. Phosphorylation on the C-terminal tail at Tyr-531 by CSK maintains the enzyme in an inactive state. PTPRC/CD45 dephosphorylates Tyr-531 leading to activation. Ultraviolet B (UVB) strongly increase phosphorylation at Thr-15 and kinase activity, and promotes translocation from the cytoplasm to the nucleus. Dephosphorylation at Tyr-420 by PTPN2 negatively regulates T-cell receptor signaling (By similarity). |
| Cellular Location: | Cytoplasm. Nucleus. Cell membrane. Present and active in lipid rafts. Palmitoylation is crucial for proper trafficking (By similarity). |
| Database Links: |
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| Synonyms: | |
| Information: | Target information shown above is from the UniProt Consortium. |
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