产品详情
简单介绍：
Anti-DDR1 (Phospho-Tyr513) Antibody
详情介绍：
Overview
| Name: | Anti-DDR1 (Phospho-Tyr513) Antibody See all DDR1 primary antibodies
|
| Description: | Rabbit polyclonal antibody to DDR1 (Phospho-Tyr513) |
| Specificity: | The antibody detects endogenous levels of DDR1 only when phosphorylated at tyrosine 513. |
| Applications: | WB, IHC |
| Dilutions: | Western blotting: 1:500~1:1000Immunohistochemistry: 1:50~1:100 |
| Reactivity: | Human |
| Immunogen: | Peptide sequence around phosphorylation site of tyrosine 513 (K-K-Y(p)-V-R) derived from Human DDR1 . |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Conjugate: | Unconjugated |
| Purification: | Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Concentration: | 1.0mg / ml |
| Formulation: | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage: | Store at -20?C / 1 year |
Target
| Function: | Tyrosine kinase that functions as cell surface receptor for fibrillar collagen and regulates cell attachment to the extracellular matrix, remodeling of the extracellular matrix, cell migration, differentiation, survival and cell proliferation. Collagen binding triggers a signaling pathway that involves SRC and leads to the activation of MAP kinases. Regulates remodeling of the extracellular matrix by up-regulation of the matrix metalloproteinases MMP2, MMP7 and MMP9, and thereby facilitates cell migration and wound healing. Required for normal blastocyst implantation during pregnancy, for normal mammary gland differentiation and normal lactation. Required for normal ear morphology and normal hearing (By similarity). Promotes smooth muscle cell migration, and thereby contributes to arterial wound healing. Also plays a role in tumor cell invasion. Phosphorylates PTPN11. |
| Tissue Specificity: | Detected in T-47D, MDA-MB-175 and HBL-100 breast carcinoma cells, A-431 epidermoid carcinoma cells, SW48 and SNU-C2B colon carcinoma cells and Hs 294T melanoma cells (at protein level). Expressed at low levels in most ***** tissues and is highest in the brain, lung, placenta and kidney. Lower levels of expression are detected in melanocytes, heart, liver, skeletal muscle and pancreas. Abundant in breast carcinoma cell lines. In the colonic mucosa, expressed in epithelia but not in the connective tissue of the lamina propria. In the thyroid gland, expressed in the epithelium of the thyroid follicles. In pancreas, expressed in the islets of Langerhans cells, but not in the surrounding epithelial cells of the exocrine pancreas. In kidney, expressed in the epithelia of the distal tubules. Not expressed in connective tissue, endothelial cells, adipose tissue, muscle cells or cells of hematopoietic origin. |
| Sequence Similarities: | Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. |
| Post-Translational Modification: | Autophosphorylated in response to fibrillar collagen binding. |
| Cellular Location: | Cell membrane. |
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