产品详情
简单介绍：
Anti-DRP1 Antibody
详情介绍：
Overview
| Name: | Anti-DRP1 Antibody See all DRP1 primary antibodies
|
| Description: | Rabbit polyclonal antibody to DRP1 |
| Applications: | ELISA, ICC, IF, IHC, IP, WB |
| Dilutions: | ELISA: 1:10,000; ELISA: 1:10,000; Immunocytochemistry: 1:100; Immunofluorescence: 1:100; Immunohistochemistry: 1:100; Immunoprecipitation: 1:200; Western Blot: 1:450 |
| Reactivity: | D. Melanogaster, Human, Mouse, Rat |
| Immunogen: | Synthetic peptide within 450-490 on human DRP1 protein. |
| Host: | Rabbit |
| Clonality: | Polyclonal |
| Conjugate: | Unconjugated |
| Concentration: | 0.55-0.68 μg/μl in antibody stabilization buffer |
| Storage: | -20 ?° C for long term storage |
Target
| Function: | Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage. Required for normal brain development, including that of cerebellum. Facilitates developmentally regulated apoptosis during neural tube formation. Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues. Plays an important role in mitochondrial fission during mitosis. Required for formation of endocytic vesicles. Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles. Required for programmed necrosis execution. |
| Tissue Specificity: | Expressed in the cerebellum and in several regions of the cerebrum and diencephalon. Strongly expressed in the cerebellar Purkinje cells and in the pontile giant neurons. |
| Sequence Similarities: | Belongs to the TRAFAC class dynamin-like GTPase superfamily. Dynamin/Fzo/YdjA family. |
| Post-Translational Modification: | Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-643 inhibits mitochondrial fission probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-622 also promotes mitochondrial fission (By similarity). |
| Cellular Location: | Cytoplasm > Cytosol. Golgi apparatus. Endomembrane system. Mitochondrion outer membrane. Peroxisome. Membrane > Clathrin-coated pit. Cytoplasmic vesicle > Secretory vesicle > Synaptic vesicle membrane. Mainly cytosolic. Translocated to the mitochondrial membrane through O-GlcNAcylation and interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Localizes to mitochondria following necrosis induction. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex (By similarity). Recruited to the mitochondrial outer membrane by interaction with MIEF1. Binds to phospholipid membranes. |
| Database Links: |
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| Synonyms: | |
| Information: | Target information shown above is from the UniProt Consortium. |
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