产品详情
简单介绍：
Anti-eIF2α (2E4) Antibody
详情介绍：
Overview
| Name: | Anti-eIF2α (2E4) Antibody See all eIF2α primary antibodies
|
| Description: | Mouse monoclonal antibody to eIF2α (2E4) |
| Specificity: | This antibody detects endogenous levels of eIF2α and does not cross-react with related proteins. |
| Applications: | WB, IF |
| Reactivity: | Human, Mouse, Rat |
| Immunogen: | Purified recombinant human eIF2α protein fragments expressed in E.coli. |
| Host: | Mouse |
| Clonality: | Monoclonal |
| Conjugate: | Unconjugated |
| Molecular Weight: | Predicted band size:38KDa Observed band size:38KDa |
| Purity: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). |
| Product Form: | Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol |
Target
| Function: | Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. |
| Sequence Similarities: | Belongs to the eIF-2-alpha family. |
| Post-Translational Modification: | Substrate for at least 4 kinases: EIF2AK1/HRI, EIF2AK2/PKR, EIF2AK3/PERK and EIF2AK4/GCN2. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation (PubMed:15207627, PubMed:18032499). Phosphorylated; phosphorylation on Ser-52 by the EIF2AK4/GCN2 protein kinase occurs in response to amino acid starvation and UV irradiation (By similarity). |
| Cellular Location: | Cytoplasmic granule. The cytoplasmic granules are stress granules which are a dense aggregation in the cytosol composed of proteins and RNAs that appear when the cell is under stress. Colocalizes with NANOS3 in the stress granules (By similarity). |
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