产品类型:

Enzyme

产品名称:

Recombinant Human Protein disulfide-isomerase protein(P4HB) (Active)

uniprot号:

规格:

20μg 100μg 250μg ￥620

基因名称:

P4HB

别名:

PDI, Cellular thyroid hormone-binding protein, Prolyl 4-hydroxylase subunit beta, p55

种属:

Homo sapiens (Human)

蛋白描述:

Partial

PubMed ID:

3034602; 3611107; 2846539; 14702039; 15489334; 1597478; 9150948; 12665801; 2079031; 1286669; 9399589; 3342239; 1699755; 10636893; 11181151; 12485997; 12095988; 12218051; 12218052; 12643545; 15158710; 14592831; 15644496; 17081065; 21269460; 24275569; 25683117; 8580850; 8672469; 10383197;

分子量:

56.6 kDa

标签信息:

N-terminal 6xHis-tagged

来源:

E.Coli

纯度:

>95% as determined by SDS-PAGE and HPLC.

缓冲液:

0.2 μm filtered PBS, pH 7.0 ,lyophilized

内毒素:

Less than 1.0 EU/μg as determined by LAL method.

生物活性:

Thiol Protein Reductase Activity is 0.001 Δ650nm/ min-2, determined by measuring the turbidity
increase at 650 nm due to insulin reduction.The activity is expressed as the ratio of the slope of a
linear part of the turbidity curve to the lag time.

Uniprot登录名:

PDIA1_HUMAN

参考文献:

This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. {ECO:0000269|PubMed:10636893, ECO:0000269|PubMed:12485997}.

氨基酸序列:

MRGSGSHHHH HH+APEEEDHV LVLRKSNFAE ALAAHKYLLV EFYAPWCGHC KALAPEYAKA AGKLKAEGSE IRLAKVDATE ESDLAQQYGV RGYPTIKFFR NGDTASPKEY TAGREADDIV NWLKKRTGPA ATTLPDGAAA ESLVESSEVA VIGFFKDVES DSAKQFLQAA EAIDDIPFGI TSNSDVFSKY QLDKDGVVLF KKFDEGRNNF EGEVTKENLL DFIKHNQLPL VIEFTEQTAP KIFGGEIKTH ILLFLPKSVS DYDGKLSNFK TAAESFKGKI LFIFIDSDHT DNQRILEFFG LKKEECPAVR LITLEEEMTK YKPESEELTA ERITEFCHRF LEGKIKPHLM SQELPEDWDK QPVKVLVGKN FEDVAFDEKK NVFVEFYAPW CGHCKQLAPI WDKLGETYKD HENIVIAKMD STANEVEAVK VHSFPTLKFF PASADRTVID

表达区域:

19-456aa

研究领域:

Signal Transduction