产品类型:

Tag/Control Antibodies

产品名称:

MYC Monoclonal Antibody

规格:

100μg 50μg ￥88

免疫原:

EQKLISEEDL (Myc) synthetic peptide conjugate to KLH

宿主:

Mouse

应用范围:

ELISA,WB,IF,IP;Recommended dilution:WB:1:500-1:5000,IF:1:100-1:300,IP:1:1000-1:1500

背景:

Myc tag is a polypeptide protein tag derived from the c-myc gene product that can be added to a protein. It can help to separate recombinant, overexpressed protein from wild type protein expressed by the host organism. Myc Tag also can be used to isolate protein complexes with multiple subunits. The Myc Tag Antibody is produced by the conjugation of a synthetic Myc tag peptide to KLH. Myc Tag Antibody can be used in various immunoassays, such as ELISA, Western blotting, immunoprecipitation, immunofluorescence, and more.

克隆类型:

Monoclonal

抗体亚型:

IgG1

纯化方式:

>95%,Protein G purified

标记方式:

Non-conjugated

保存缓冲液:

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

产品提供形式:

Liquid

储存条件:

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

图片:

WB:Mouse anti Myc-tagged fusion protein Monoclonal antibody at 1.6ug/ml
Line 1 : Recombinant Myc-tagged fusion protein at 50ng
Line 2 : Recombinant Myc-tagged fusion protein at 25ng
Line 3 : Recombinant Myc-tagged fusion protein at 12.25ng
Line 4 : Recombinant Myc-tagged fusion protein at 6.25ng
Line 5 : Recombinant Myc-tagged fusion protein at 3.125ng
Line 6 : Recombinant Myc-tagged fusion protein at 1.5625ng
Secondary
Goat polyclonal to Mouse IgG at 1/50000 dilution
Predicted band size : 50kd
Observed band size : 50kd

Immunofluorescence staining of transfected HEK293 cells with CSB-MA000041M0m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG (H+L).

Immunoprecipitating MYC-tag with transfected HEK293
Lane 1: Mouse control IgG(1µg)instead of CSB-MA000041M0m in transfected HEK293 whole cell lysate.
For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000041M0m(6µg)+ transfected HEK293 whole cell lysate(1mg)
Lane 3: Transfected HEK293 whole cell lysate (10µg)

 

别名:

bHLHe39, c Myc, MRTL, MYC, Myc proto oncogene protein, MYC tag, Proto oncogene c Myc, Transcription factor p64