产品类型:

RT-PCR Primers

产品名称:

MCM3

英文名称:

MCM3

规格:

100 reactions ￥500

种属反应性:

Human

简介:

Human MCM3 Primer Pair for ChIP

应用领域:

ChIP

PCR产物长度:

222 bp

储存条件:

The Primer Pair is stable for up to one year at -20° C in a non-frost free freezer. Avoid repeated freeze-thaw cycles.

质量控制:

 

MCM3 Primers were tested on DNA isolated from cross-linked 293T cells using formaldehyde and extracted with genomic DNA extraction kit. Real-time PCR was performed in triplicate on a serial dilution of input DNA (100ng, 20ng, 4ng, 0.8ng, 0.16ng and 0.032ng) using a real-time PCR detection system and SYBR^®Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R²) were calculated based on the corresponding threshold cycle (C_T) of each dilution sample during 40 cycles of real-time PCR (95 °C denaturation for 15 sec, 60 °C anneal/extension for 45 sec).

引物退火/延伸:

60 °C anneal/extension for 45 sec