企业信息
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  • 入驻时间: 2008-08-20
  • 联系人:客户经理
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  • Email:fuwu@bioleaf.com
产品详情
  • 产品名称:STAT5B

  • 产品型号:
  • 产品厂商:其它品牌
  • 产品价格:0
  • 折扣价格:0
  • 产品文档:
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简单介绍:
STAT5B
详情介绍:
  • 产品类型:
    RT-PCR Primers
  • 产品名称:

    STAT5B

  • 英文名称:

    STAT5B

  • 规格:
    ¥500
  • 种属反应性:
    Human
  • 简介:
    Human STAT5B Primer Pair for ChIP
  • 应用领域:
    ChIP
  • PCR产物长度:
    140 bp
  • 储存条件:
    The Primer Pair is stable for up to one year at -20° C in a non-frost free freezer. Avoid repeated freeze-thaw cycles.
  • 质量控制:

    STAT5B Primers were tested on DNA isolated from cross-linked MCF-7 cells using formaldehyde and extracted with genomic DNA extraction kit. Real-time PCR was performed in triplicate on a serial dilution of input DNA (100ng, 20ng, 4ng, 0.8ng, 0.16ng and 0.032ng) using a real-time PCR detection system and SYBR® Green reaction mix. The PCR amplification efficiency (E) and correlation coefficient (R2) were calculated based on the corresponding threshold cycle (CT) of each dilution sample during 40 cycles of real-time PCR (95 °C denaturation for 15 sec, 60 °C anneal/extension for 45 sec).

  • 引物退火/延伸:
    60 °C anneal/extension for 45 sec
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