产品类型:

RT-PCR Primers

产品名称:

Human PRKAG1 Primer Pair

英文名称:

Human PRKAG1 Primer Pair

规格:

25T

简介:

Human PRKAG1 Primer Pair for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.

长度:

998bp

登录号:

NM_002733

应用领域:

RT-PCR

储存条件:

The Primer Pair is stable for up to one year at
-20° C in a non-frost free freezer。
Avoid repeated freeze-thaw cycles.

质量控制:

Primer Pairs for RT-PCR provide convenient
primers for analyzing the expression of specific
mRNAs or got specific PCR fragment by RT-PCR. Each Primer Pair includes a 5´ and 3´ oligonucleotide primer provided in individual vials at 50μM ready for addition to the amplification reaction. The Primer Pairs have been confirmed in two-step conventional RT-PCR using human esophageal carcinomaEC109 cells total RNA.
PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate.

技术指导:

• Thaw all reagents completely on ice before use. • Use either random primers or oligo (dT) for reverse transcription. • All PCR reactions should be assembled on ice. • The recommended annealing