产品详情
简单介绍:
Human JAK1 Primer Pair 3
详情介绍:
RT-PCR Primers
Human JAK1 Primer Pair 3
Human JAK1 Primer Pair 3
Human JAK1 Primer Pair 3 for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.
746bp
NM_002227
RT-PCR
The Primer Pair is stable for up to one year at
-20° C in a non-frost free freezer。
Avoid repeated freeze-thaw cycles.
-20° C in a non-frost free freezer。
Avoid repeated freeze-thaw cycles.
Primer Pairs for RT-PCR provide convenient primers for analyzing the expression of specific mRNAs or got specific PCR fragment by RT-PCR. Each Primer Pair includes a 5´ and 3´ oligonucleotide primer provided in individual vials at 50μM ready for addition to the amplification reaction. The Primer Pairs have been confirmed in two-step conventional RT-PCR using human esophageal carcinoma EC109 cells total RNA.
PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate.
PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate.
• Thaw all reagents completely on ice before use.
• Use either random primers or oligo (dT) for reverse transcription.
• All PCR reactions should be assembled on ice.
• The recommended annealing
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