产品类型:

RT-PCR Primers

产品名称:

Rat IGFBP5 Primer Pair 1

英文名称:

Rat IGFBP5 Primer Pair 1

规格:

25T

简介:

Rat IGFBP5 Primer Pair 1 for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.

长度:

779bp

登录号:

NM_012817

应用领域:

RT-PCR

储存条件:

The Primer Pair is stable for up to one year at -20° C in a non-frost free freezer。
Avoid repeated freeze-thaw cycles.

质量控制:

Primer Pairs for RT-PCR provide convenient primers for analyzing the expression of specific mRNAs or got specific PCR fragment by RT-PCR. Each Primer Pair includes a 5´ and 3´ oligonucleotide primer provided in individual vials at 50μM ready for addition to the amplification reaction. The Primer Pairs have been confirmed in two-step conventional RT-PCR using rat heart, liver and kidney total RNA.
PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate.

技术指导:

• Thaw all reagents completely on ice before use. • Use either random primers or oligo (dT) for reverse transcription. • All PCR reactions should be assembled on ice. • The recommended annealing