产品详情
简单介绍：
Aspartate Aminotransferase (AST, GOT)
详情介绍：
| Product Name | Aspartate Aminotransferase (AST, GOT) |
| Source | Porcine Heart |
| Catalogue Number | 300-20 |
| Purity | Purified |
| Form | Lyophilized |
| Activity | > 100 U/mg (Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the transamination of one micromole of L-aspartate to alpha-ketoglutarate forming L-glutamate and oxaloacetate per minute at 37°C and pH 7.8 |
| Protein | > 0.7 mg protein/mg solid (A280) Report mg protein/mg solid (Coomassie) |
| Specific Activity | > 110 U/mg protein (A280) Report U/mg protein (Coomassie) |
| Contaminants | CPK: < 0.1% GPT/ALT: < 0.1% LDH: < 0.1% GLDH: < 0.1% MDH: < 0.01% Protease: < 0.1% Ammonia: < 0.005 micromole/mg |
| Formulation | Lyophilized from sodium phosphate and pyridoxal-5-phosphate, pH 7.0. |
| Appearance | Yellow-green powder |
| Reconstitution | > 1 mg/mL in tris buffered saline, 1% BSA, pH 8.0. |
| Isoform | Cytosolic |
| Storage | -20°C |
| Recertification | 3 years |
| Application | Control, Calibrator, Research, Manufacturing |
| Synonyms | Aspartate Transaminase, glutamate oxaloacetate transaminase, AST, GOT, sGOT, AspAT, ASAT, AAT, serum glutamic oxaloacetic transaminase |
| Molecular Weight | ~92,000 (Feliss and martinez-Carrion 1970) |
| CAS Number | 9000-97-9 |
| E.C. Number | 2.6.1.1 |
| Gene | GOT1 |
| Accession No | P00503 |
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