产品详情
简单介绍：
Butyrylcholinesterase (BChE)
详情介绍：
| Product Name | Butyrylcholinesterase |
| Source | Equine Serum |
| Catalogue Number | 130-10 |
| Purity | Highly Purified |
| Form | Lyophilized |
| Activity | > 50 U/mg (Siemens Dimension® Clinical Chemistry System) |
| Unit Definition | One unit will catalyze the hydrolysis of one micromole of butyrylthiocholine to thiocholine and butyrate per minute at 37°C. |
| Protein | > 0.1 mg protein/mg (A280nm, E = 1.36) |
| Specific Activity | > 300 U/mg protein (Typically > 900 U/mg protein) |
| Appearance | White to pale, grey-green powder |
| Reconstitution | Reconstitute at 5 mg/mL using 50 mM Tris-HCl pH 7.3 - 7.5. |
| Solubility | Clear, colorless to slightly green solution (1 mg/mL in 0.9% saline) |
| Recertification | 4 years |
| Storage | 2-8°C |
| Synonyms | BCHE, BuChE, PCHE, pseudocholinesterase, plasma cholinesterase, Acylcholine acyl-hydrolase, Choline esterase, butyryl |
| Application | Diagnostic Manufacturing, Clinical Research |
| CAS Number | 9001-08-5 |
| E.C. Number | 3.1.1.8 |
| Gene | BCHE |
| Accession No | P06276 (human, 29 – 602), Q9N1N9 (equine) |
| Reaction | Acylcholine + H2O = choline + carboxylic acid |
| Kinetics | KM=18 µM (butyrylthiocholine, 25 degrees Celsius) |
| Activators | Ca2+ |
| Inhibitors | Hg2+, EDTA, diisopropylfluorophosphate |
| Optimum pH | 7 - 8 |
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