Cytodiagnostics 90nm Maleimide-activated Gold NanoUrchin Conjugation Kit has been optimized for high efficiency one-step conjugation of thiol-containing ligands to gold Gold NanoUrchins.
The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanourchins is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated Maleimide gold nanourchins supplied in the kit.
Kit Components
- 90nm Maleimide-Activated Gold NanoUrchins (lyophilized)
- Protein Resuspension Buffer
- Reaction Buffer
- Quencher Solution
Features & Benefits
- Results in covalently bound ligand and more stable conjugate.
- Fast and convenient one-step conjugation reaction with no pre-activation requirements.
- Spacer between the gold surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, a common problem seen in conjugates prepared by passive adsorption.
- Precisely engineering surface coating that reduces non-specific protein binding in biological assays.
Gold Nanoparticle Specifications
Surface functionality: Maleimide (~ 1 Maleimide group/nm2) (10kDa PEG-spacer between gold surface and Maleimide-group)
Core diameter: 90nm
Concentration: OD=20 (when dissolved to a final volume of 100ul)
Particles per ml: ~1.07E+11 (when dissolved to a final volume of 100ul)
Absorbance (lambda max): 630nm
Diameter | Peak SPR Wavelength (nm) | NPS/ml* | Wt. Conc (mg/ml)* | Molar Ext (M-1cm-1) | Size Dispersity (+/-nm) | Particle Volume (nm3)# | Surface Area (nm2)# | Surface/ Volume Ratio# | Particle Mass (g) | Molar Mass (g/mol) | Molar Concentration* |
90 | 630 | 5.37E+09 | 3.97E-02 | 1.12E+11 | <8% | 3.82E+05 | 2.54E+04 | 0.067 | 7.40E-15 | 4.46E+09 | 8.92E-12 |
| *All concentrations as indicated at an optical density of 1 (OD=1), # Data is approximated based upon a spherical nanoparticle. | |||||||||||
