Native Chlamydia trachomatis antigens from clarified cell lysate.
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Chlamydia trachomatis antigen
Chlamydia trachomatis is propagated to maximum density in McCoy cells under Category 2 safety conditions. Infected cells are carefully washed and disrupted into buffered saline (PBS), subjected to 3 freeze/thaw cycles and centrifuged to remove cellular debris. Chlamydia antigen production is scalable from 10 mL to several litres and is optimised by the multiplicity of infection and by harvesting time.
Inactivation is by heat treatment and the addition of triton x-100 to render products non-infectious and improve stability. All preparations are tested as not infectious by double passage on McCoy cell under optimal growth conditions.
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