Dengue virus NS1 antibody (serotype 1) – clone OB4

Human IgM anti Dengue virus NS1 antibody is specific for the NS1 protein of Dengue virus serotype 1. It demonstrates negligible cross-reactivity with NS1 proteins from other Dengue virus serotypes or with other flaviviruses. No cross-reactivity is seen with Chikungunya virus proteins (E1, E2 and C).

This human IgM anti Dengue virus serotype 1 NS1 monoclonal antibody has been prepared by chimerization from the mouse monoclonal antibody (clone OB4). The original variable domains of this antibody have been retained, whilst the constant regions have been replaced with human IgM.

The antibody is designed to provide a control for assays in which human serum is tested for antibodies specific for Dengue serotype 1 NS1 protein, which will result from the immune response following an infection with Dengue virus. The antibody has been tested in ELISA using recombinant Dengue virus serotype 1 NS1 protein as the target antigen.

The NS1 protein is a major non-structural protein expressed by the Dengue Virus. The NS1 monomer is a glycosylated protein of approximately 45kD, which associates with lipids and forms a homodimer inside infected cells. It is necessary for viral replication, and is also secreted into the extracellular space as a hexameric lipoprotein particle, which is involved in immune evasion and pathogenesis by interacting with components from both the innate and adaptive immune systems, as well as other host factors. NS1 is one of the major antigenic markers for viral infection with Dengue.

With more than one-third of the world’s population living in areas at risk of transmission, dengue infection is a leading cause of illness and death in the tropics and subtropics (ref WHO for Guidelines for Diagnosis, Treatment, Prevention & Control, 2009) As many as 100 million people are infected yearly. Dengue is caused by any one of four related viruses transmitted by mosquitoes. There are no vaccines available to prevent infection with dengue virus.

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