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  • 产品名称：Oligo-Abeta-pE3 - 218 511

  • 产品型号：218 511
  • 产品厂商：其它品牌
  • 产品价格：35000
  • 折扣价格：0
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简单介绍：
Oligo-Abeta-pE3 - 218 511
详情介绍：
Cat. No. 218 511 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Applications WB: 1 : 500 (see remarks) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 gallery  
IHC-P/FFPE: 1 : 100 (see remarks) gallery  
ELISA: yes (see remarks)
Important note for users The mouse monoclonal antibody clone 9D5, Cat. No. 218 511, is patented (patent application PCT/EP2011/002739). By purchasing this antibody the customer acquires rights to use this product for research purposes only. Any diagnostic and therapeutic in vitro or in vivo use is explicitly excluded.
Clone 9D5
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 3 to 38 from human Oligo-Abeta-pE3 (UniProt Id: P05067)
Epitop Epitop: AA 3 to 38 from human Oligo-Abeta-pE3 (UniProt Id: P05067)
Reactivity Reacts with: human (P05067), mouse (P12023).
Other species not tested yet.
Specificity Recognizes specific oligomeric structures formed preferentially by Abeta-pE3.
Remarks

WB: We recommend the Invitrogen NativePAGE system in combination with PVDF blotting membranes. Boil membrane after blotting for 3min.
Peptide preparation: Synthetic Abeta peptides were monomerized in 70 % formic acid, and the solvent was evaporated in a speed-vac immediately. Prior to each experiment, peptides were dissolved in 0.3 % ammonia, underwent ultrasonic treatment, and were further diluted to an end concentration of 0.15 % ammonia.

IHC-P: Formic acid treatment required.

ELISA: Suitable as capture antibody for sandwich-ELISA with cat. no. 218 011BT as detector antibody (protocol for sandwich-ELISA).
Datasheet 218_511.pdf
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