产品详情
简单介绍:
Bassoon - 141 003
详情介绍:
Cat. No. 141 003 | 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use. |
Applications |
WB: 1 : 100 up to 1 : 1000 (AP staining) (see remarks) IP: not tested yet ICC: 1 : 200 up to 1 : 2000 gallery IHC: 1 : 200 gallery IHC-P/FFPE: 1 : 500 gallery ELISA: yes (see remarks) |
Immunogen | Recombinant protein corresponding to AA 3608 to 3938 from rat Bassoon (UniProt Id: O88778) |
Reactivity |
Reacts with: rat (O88778), mouse (O88737). No signal: chicken. Other species not tested yet. |
Specificity | Specific for bassoon. |
matching control protein/peptide | 141-0P |
Remarks |
WB: Due to its large size, bassoon requires special gel-electrophoresis and Western blot protocols for visualization by immunoblotting.Excellent results can be obtained with the 4-12% TRIS-glycine gradient gels of anamed. ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 141 021 as capture antibody (protocol for sandwich-ELISA). |
Datasheet | 141_003.pdf |
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