产品详情
简单介绍：
Synaptotagmin 1 lumenal domain - 105 103CpH
详情介绍：
| Cat. No. 105 103CpH |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with CypHer5E. Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
WB: N/A (see remarks) IP: N/A ICC: 1 : 100 up to 1 : 500 gallery IHC: not tested yet IHC-P/FFPE: not tested yet |
| Label | CypHer5E |
| Immunogen | Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096) |
| Reactivity |
Reacts with: rat (P21707), mouse (P46096). Other species not tested yet. For unknown reasons antibodies raised against the luminal N-terminus of Synaptotagmin 1 show a strong preference for the rat protein. |
| Specificity | Specific for synaptotagmin 1, no cross-reactivity to synaptotagmin 2. |
| matching control protein/peptide | 105-10P |
| Remarks |
This antibody can be used for labeling of recycling synaptic vesicles by adding to living neurons or as a marker for exocytosis in isolated nerve terminals. WB: This antibody is intended to be used for direct labeling of recycling synapses in primary neuronal cultures. The pH sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen. |
| Datasheet | 105_103cph.pdf |
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