产品详情
简单介绍：
Synaptotagmin 1 lumenal domain - 105 103AF
详情介绍：
| Cat. No. 105 103AF |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen, fluorescence-labeled with AcidiFluor Orange . Rabbit serum albumin was added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Either add 1:1 (v/v) glycerol, then aliquot and store at -20°C until use, or store aliquots at -80°C without additives. Reconstitute immediately upon receipt! Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye. |
| Applications |
WB: N/A IP: N/A ICC: 1 : 100 up to 1 : 300 (see remarks) gallery IHC: not tested yet IHC-P/FFPE: not tested yet |
| Label | AcidiFluor Orange |
| Immunogen | Synthetic peptide corresponding to AA 1 to 8 from mouse Synaptotagmin1 (UniProt Id: P46096) |
| Reactivity |
Reacts with: rat (P21707). Other species not tested yet. |
| Specificity | specific for Synaptotagmin 1 |
| matching control protein/peptide | 105-10P |
| Remarks |
ICC: This antibody is intended for direct labeling of recycling synaptic vesicles in living primary neuronal cultures. The pH-sensitive dye regaines its fluorescence after the reacidification of the synaptic vesicle lumen. |
| Datasheet | 105_103af.pdf |
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