Description
The ViPrimePLUS Streptococcus agalactiae qPCR Kit is a Taqman probe-based real-time PCR assay for the detection of Streptococcus agalactiae genomes in clinical swab samples (e.g. vaginal swab, urine and serum) and veterinary products such as milk.
Streptococcus agalactiae is a beta-hemolytic bacterium that belongs to group B streptococci (GBS). In veterinary, it is the main cause of mastitis in dairy cattle, resulting in the drop in milk yield and quality. It is also a human pathogen where it colonizes the urinary and gastrointestinal tract of healthy individuals as well as the vaginal area of women. Therefore, this leads to a high risk of neonatal infection transmitted from the infected mothers during pregnancy or delivery. In neonates, 1-2% of them will develop invasive group B streptococcal diseases, such as pneumonia, bacteremia and meningitis. There are increasing cases of invasive infection reported in *****s particularly those with underlying medical conditions and elderly. Clinical manifestation of the disease is linked to the site of infections, such as cirrhosis, cardiovascular, genitourinary, renal and neurological deficits. Therefore early detection enables early treatment and cure.
ViPrimePLUS Streptococcus agalactiae qPCR Kit is intended for research use only.
Kit Component
- S. agalactiae Primers and Probe Mix (S. agalactiae PPM)
- Positive Control
- Internal Extraction Control Primers and Probe Mix (IEC PPM)
- Internal Extraction Control (IEC DNA)
- Nuclease Free Water
- Template Preparation Buffer
Product Specification
| Technology | Taqman probe-based real-time PCR assay |
| Type of Nucleic Acid Kit | DNA |
| Kit Storage | Shipped in room temperature, store at -20℃ on arrival |
| Sample Material |
• Clinical samples (e.g. vaginal swab, urine and serum) • Veterinary products |
| Gene Target | cAMP factor gene (cfb). |
| Detection Limit | 10 to <100 copies per reaction |
| Sensitivity & Specificity | Ct value 28±3 with probability of 95% |
| Controls Included |
• Internal Extraction Control (IEC) as inhibition control • Positive control |
| Channels |
• FAM channels detect pathogen amplicons • VIC/HEX channel detects IEC amplicons |
Ordering Information
| Catalog No | Description | Pack Size |
| QM2058 | ViPrimePLUS Streptococcus agalactiae qPCR Kit | 150 reactions |
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Manual
ViPrimePLUS Streptococcus agalactiae qPCR Kit
