Alkyne Phosphoramidite, 5'-terminal
| Cat. # | Quantity | Price | Lead time | Buy this product |
|---|---|---|---|---|
| 22260 | 250 mg | 90.00$ | in stock | |
| 42260 | 1 g | 190.00$ | in stock | |
| 62260 | 5 g | 690.00$ | in stock | |
| 82260 | 10 g |
 1190.00$
|
10 days |
Phosphoramidite for the synthesis of oligonucleotides with 5'-terminal alkyne for Click Chemistry.
This alkyne amidite has several advantages over 5’-hexynyl phosphoramidite, 5'-butynyl-CEP, and other 5'-terminal alkyne phosphoramidites. First, it is solid compound which is easier to handle and dispense. And due to its structure, it is also more stable in solution, and has longer shelf life
Diluent for this phosphoramidite is acetonitrile, 5 min coupling time is recommended. Because this amidite does not contain 5'-terminal DMT group, no 5'-deprotection needed. Oligonucleotides should be deblocked under standard conditions, and purified by PAGE, or ion exchange HPLC.
Oligonucleotides with this modification are ideal for the use in Click Chemistry (see our recommended protocol).
Customers also purchased with this product
Cyanine5 azide
Cyanine5 azide is a reagent for Click Chemistry labeling with Cyanine5, a popular fluorescent dye.3-Ethynyl perylene
Alkyne derivative of ultra-bright and photostable perylene fluorophore.Alkyne Amidite, hydroxyprolinol
Acetylenic amidite for the incorporation of acetylene groups into oligos.General properties
| Appearance: | colorless solid |
| Molecular weight: | 409.50 |
| Molecular formula: | C21H36N3O3P |
| IUPAC name: | trans-4-(5-Hexynoylamino)cyclohexyloxy-N,N-diisopropylamino-2-cyanoethyloxyphosphine |
| Solubility: | good in acetonitrile and dichloromethane |
| Quality control: | NMR 1H (95%) and 31P, HPLC-MS |
| Storage conditions: | Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | Download |
Oligo synthesis details
| Coupling conditions: | Standard coupling, identical to normal nucleobases |
| Cleavage conditions: | standard deprotection |
| Deprotection conditions: | No deprotection required. Compatible with standard deprotection reagents. |
Product citations
- Taskova, M.; Uhd, J.; Miotke, L.; Kubit, M.; Bell, J.; Ji, H.P.; Astakhova, K. Tandem Oligonucleotide Probe Annealing and Elongation To Discriminate Viral Sequence. Analytical Chemistry, 2017, 89(8), 4363–4366. doi: 10.1021/acs.analchem.7b00646
- Aparin, I.O.; Farzan, V.M.; Veselova, O.A.; Chistov, A.A.; Podkolzin, A.T.; Ustinov, A.V.; Shipulin, G.A.; Formanovsky, A.A.; Korshun, V.A.; Zatsepin, T.S. 1-Phenylethynylpyrene (PEPy) as a novel blue-emitting dye for qPCR assay. Analyst, 2016, 141, 1331–1338. doi:10.1039/c5an01767j
- Astakhova, I.K.; Santhosh Kumar, T.; Campbell, M.A.; Ustinov, A.V.; Korshun, V.A.; Wengel, J. Branched DNA nanostructures efficiently stabilised and monitored by novel pyrene-perylene 2'-alpha-L-amino-LNA FRET pairs. Chemical Communications, 2013, 49(5), 511-511. doi: 10.1039/c2cc37547h
