| Product Details | |
| Product Name | Anti-Histone H3.3 G34R Rabbit Monoclonal Antibody [RM240] |
| Product Description | Rabbit monoclonal to Histone H3.3 G34R; Histone H3.3 G34R Mutant |
| Catalog No. | 31-1120-00 |
| Clone Name | RM240 |
| Product Data Sheet | Anti-Histone H3.3 G34R Mutant Rabbit Monoclonal Antibody Data Sheet RM240 |
| Specificity | RM240 reacts to the Histone H3.3 G34R mutant. No cross reactivity with wild type Histone H3.3 . |
| Immunogen | A peptide corresponding to Histone H3.3 G34R mutant |
| Application | Western Blot ELISA Immunohistochemistry Immunocytochemistry Chromatin IP |
| Species Reactivity | All |
| Conjugate | None |
| Intended Use | Research Use Only |
| . | |
| Properties | |
| Form | Liquid |
| Storage Instructions | Store at -20.0°C |
| Stability | Stable for 1 Year at -20.0°C from date of receipt |
| Storage Buffer | 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide |
| Volume/Size | 50 ug |
| Concentration | 1.0 mg/mL |
| Purity | Protein A affinity purified from an animal origin–free culture supernatant |
| Clone Type | Monoclonal |
| Isotype | Rabbit IgG |
| Usage | Western Blot: 0.5 ug/mL – 2 ug/mL; ELISA: 0.5 ug/mL - 2 ug/mL; Immunohistochemistry (IHC): 0.5 – 5 ug/mL; Immunocytochemistry (ICC): 0.5 – 5 ug/mL. |
| References for Histone H3.3 G34R Mutant antibody [RM240] | Pathania et al. H3.3K27M Cooperates with Trp53 Loss and PDGFRA Gain in Mouse Embryonic Neural Progenitor Cells to Induce Invasive High-Grade Gliomas Cancer Cell (2017)10.1016/j.ccell.2017.09.014. WB; Human. Read more (PubMed: 29107533) Yamamoto H.et al. Diagnostic utility of histone H3.3G34 W, G34R, and G34 V mutant-specific antibodies for giant cell tumors of bone. Human Pathology (2017)10.1016/j.humpath.2017.11.020 IHC; Human. Read more (PubMed: 29241742) |
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| Product Images | |
 | Western Blot analysis of cell lysates prepared from |
 | Immunohistochemical staining of formalin fixed and |
 | IHC of clinically validated FFPE Glioblastoma tumor Image courtesy of Dr. Sebastian Brandner, UCL |
 | IHC of clinically validated FFPE Glioblastoma tumor Image courtesy of Dr. Sebastian Brandner, UCL |
 | ICC staining of primary human cell lines expressing Image courtesy of Dr. Nada Jabado, McGill University, |
